Sulfolobus acidocaldarius

iModulonDB

iModulons database S. acidocaldarius

Independent Component Analysis of Sulfolobus acidocaldarius Transcriptomes

ChIP-Seq Occupancy Mapping of the Archaeal Transcription Machinery

Genome-wide occupancy studies for RNA polymerases and their basal transcription factors deliver information
about transcription dynamics and the recruitment of transcription elongation and termination
factors in eukaryotes and prokaryotes. The primary method to determine genome-wide occupancies is
chromatin immunoprecipitation combined with deep sequencing (ChIP-seq). Archaea possess a transcription
machinery that is evolutionarily closer related to its eukaryotic counterpart but it operates in a

Dr. Abhrajyoti Ghosh

Bose Institute

Dr. Danna R Gifford

University of Manchester

Veerke De Kock

Vrije Universiteit Brussel

example-protocol_long_saci-acetic-acid-synchronisation.pdf

Synchronisation of S. acidocaldarius with Acetic Acid

This protocol describes how to synchronise a S. acidocaldarius cell culture. The first step is to administer acetic acid to arrest the cells in a G2-like state. Once washed, the culture proceeds synchronously through division and DNA replication for about two cell cycles.

20240312--cezanne--scanning-electron-microscopy-to-characterize-cell-shape-in-sulfolobus-acidocaldarius.pdf

Scanning Electron Microscopy to characterize cell shape in Sulfolobus acidocaldarius

This protocol describes the preparation of Sulfolobus acidocaldarius cells for imaging in Scanning Electron Microscopy (SEM). Sulfolobus acidocaldarius is a species of thermoacidophilic archaea. Due to their small cell size (in the micron range), morphological studies rely on high-resolution imaging methods like super-resolution light microscopy or electron microscopy. Electron microscopy is the method of choice when imaging the finest details of cells ultrastructure.