Protein Extraction and Western Blotting for Sulfolobus

This protocol describes the step-by-step methodology for extracting proteins from cells and analysing them via Western blot. The workflow includes cell lysis, protein quantification, SDS-PAGE electrophoresis, protein transfer to nitrocellulose, antibody incubation, and visualisation using Licor technology. The protocol ensures reproducible results and is adaptable for whole proteome analysis. Alternative lysis buffers, such as RIPA or TK150, may be used depending on downstream applications. The method enables efficient protein detection and quantification, critical for studying protein expression and modifications.