Ethanol Fixation Protocol for Sulfolobus acidocaldarius

This protocol describes the ethanol fixation of Sulfolobus acidocaldarius, which is used for immunofluorescence and flow cytometry applications. Ethanol fixation is a widely used method to preserve cell morphology and antigenicity while ensuring long-term sample storage. Alternative fixation methods include methanol and formaldehyde fixation, which offer different advantages depending on the downstream application. This protocol details the step-by-step preparation of ethanol-fixed samples, ensuring optimal preservation for cytometric and imaging analyses. While ethanol fixation is less permanent than e.g., formaldehyde fixation, samples remain stable for several months at 4°C.