Posttranscriptional regulation actuated by small RNAs (sRNAs) plays essential roles in a wide variety of
cellular processes, especially in stress responses and environmental signaling. Hundreds of sRNAs have
recently been discovered in archaea using genome-wide approaches but the molecular mechanisms of only a
few have been characterized experimentally. Here, we describe how to build sRNA sequencing libraries
using size-selected total RNA in the model archaeon, Haloferax volcanii, to provide a tool to further
characterize sRNAs in archaea.
Gelsinger, D.R., DiRuggiero, J. (2022). Small RNA-Sequencing Library Preparation for the Halophilic Archaeon Haloferax volcanii. In: Ferreira-Cerca, S. (eds) Archaea. Methods in Molecular Biology, vol 2522. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2445-6_15
Link to chapter: https://link.springer.com/protocol/10.1007/978-1-0716-2445-6_15